PTN Polyklonaler Antikörper

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• KD/KO Validated

PTN Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 27117-1-AP

Synonyme

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 27117-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27117-1-AP (PTN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 27117-1-AP

  U-87 MG (WT and PTN KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of U-87 MG using 27117-1-AP

  U-87 MG lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human brain using 27117-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27117-1-AP (PTN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 27117-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27117-1-AP (PTN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

27117-1-AP bindet in WB, IHC, IF, ELISA PTN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTN fusion protein Ag26004
• Vollständiger Name: pleiotrophin
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC005916
• Gene symbol: PTN
• Gene ID (NCBI): 5764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pleiotrophin (PTN) is a multifunctional, cationic, glycosaminoglycan-binding cytokine and growth factor involved in numerous physiological and pathological processes, including tissue repair and inflammation-related diseases. PTN is highly upregulated in the brain in different disorders characterized by overt neuroinflammation such as neurodegenerative diseases, drug addiction, traumatic injury, and ischemia. PTN is important for CNS repair and for survival and differentiation of dopaminergic neurons.

Publikationen

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao
human	WB	Oncol Rep; Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR‑384/pleiotrophin axis.; Authors - Yuanyuan Yao
human	WB	Oral Dis; PTN inhibited chondrogenic differentiation potential of dental pulp stem cells; Authors - Chang Liu
	IF	Cell Rep; Dissecting the spermatogonial stem cell niche using spatial transcriptomics; Authors - Shreya Rajachandran