PTK2B Monoklonaler Antikörper

PTK2B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10G5

Kat-Nr. : 67141-1-Ig

Synonyme

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

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Galerie der Validierungsdaten

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  WB analysis using 67141-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PTK2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67141-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PTK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Hausschwein-Hirngewebe, K-562-Zellen, Maushirngewebe, Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67141-1-Ig bindet in WB, ELISA PTK2B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTK2B fusion protein Ag11487
• Vollständiger Name: PTK2B protein tyrosine kinase 2 beta
• Berechnetes Molekulargewicht: 1009 aa, 116 kDa
• Beobachtetes Molekulargewicht: 112-115 kDa
• GenBank-Zugangsnummer: BC042599
• Gene symbol: PTK2B
• Gene ID (NCBI): 2185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.