PTH2R Polyklonaler Antikörper

PTH2R Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14166-1-AP

Synonyme

parathyroid hormone 2 receptor, PTH2 receptor, PTH2R, PTHR2

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Galerie der Validierungsdaten

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  WB analysis of L02 using 14166-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14166-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14166-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14166-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14166-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14166-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of BxPC-3 using 14166-1-AP

  1X10^6 BxPC-3 cells were stained with 0.2ug PTH2R antibody (14166-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, BxPC-3-Zellen, humanes Hodengewebe, Maushodengewebe, Mauslebergewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14166-1-AP bindet in WB, IHC, FC,ELISA PTH2R und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTH2R fusion protein Ag5364
• Vollständiger Name: parathyroid hormone 2 receptor
• Berechnetes Molekulargewicht: 550 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62-66 kDa
• GenBank-Zugangsnummer: BC036811
• Gene symbol: PTH2R
• Gene ID (NCBI): 5746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.