PTGS1 Monoklonaler Antikörper

PTGS1 Monoklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12G7

Kat-Nr. : 67346-1-Ig

Synonyme

COX 1, COX1, COX3, Cyclooxygenase 1, PCOX1, PGG/HS, PGH synthase 1, PGHS 1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, PTGHS, PTGS1

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Galerie der Validierungsdaten

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  WB analysis of L02 using 67346-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood leukocyte using 67346-1-Ig

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67346-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67346-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67346-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67346-1-Ig (PTGS1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A431-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67346-1-Ig bindet in WB, IF,ELISA PTGS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTGS1 fusion protein Ag28280
• Vollständiger Name: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Berechnetes Molekulargewicht: 599 aa, 69 kDa
• Beobachtetes Molekulargewicht: 60-72 kDa
• GenBank-Zugangsnummer: BC029840
• Gene symbol: PTGS1
• Gene ID (NCBI): 5742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.