PTGES3 Monoklonaler Antikörper

PTGES3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

3C11D11

Kat-Nr. : 67736-1-Ig

Synonyme

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

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Galerie der Validierungsdaten

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  WB analysis using 67736-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67736-1-Ig (PTGES3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67736-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67736-1-Ig (PTGES3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67736-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67736-1-Ig (PTGES3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES3 antibody (67736-1-Ig, Clone: 3C11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES3 antibody (67736-1-Ig, Clone: 3C11D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67736-1-Ig bindet in WB, IHC, IF, ELISA PTGES3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTGES3 fusion protein Ag7870
• Vollständiger Name: prostaglandin E synthase 3 (cytosolic)
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC003005
• Gene symbol: PTGES3
• Gene ID (NCBI): 10728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Publikationen

Species	Application	Title
human	WB	Int Immunopharmacol; High expression of PTGES3 is an independent predictive poor prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma.; Authors - Pengxiang Gao
human	WB	Cell Death Discov; METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition; Authors - Fangfang Bi