PTBP2 Polyklonaler Antikörper

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• KD/KO Validated

PTBP2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55186-1-AP

Synonyme

brPTB, PTB, PTB like protein, PTBLP, PTBP2

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55186-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 55186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 55186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

55186-1-AP bindet in WB, IP, IHC, IF, ELISA PTBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: polypyrimidine tract binding protein 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: NM_021190
• Gene symbol: PTBP2
• Gene ID (NCBI): 58155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTBP2, also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, PTBP2 plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. (PMID:11003644) . Protein may exist in a dimeric form with a MW of 110-120 kDa (PMID: 20064465). This antibody is specific to PTBP2.

Publikationen

Species	Application	Title
human	WB	Cell Rep; PTBP1 and PTBP2 Repress Nonconserved Cryptic Exons.; Authors - Jonathan P Ling ; KD Validated
human,mouse	WB	Mol Cell Biol; RBM4 modulates radial migration via alternative splicing of Dab1 during cortex development.; Authors - Dhananjaya D
mouse	WB,IF,IP	Nat Commun; Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr; Authors - Saeede Salehi ; KD Validated