PSMG3 Polyklonaler Antikörper

PSMG3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15601-1-AP

Synonyme

C7orf48, hPAC3, PAC 3, PAC3, PSMG3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15601-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15601-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15601-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15601-1-AP bindet in WB, IHC, ELISA PSMG3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMG3 fusion protein Ag7984
• Vollständiger Name: proteasome (prosome, macropain) assembly chaperone 3
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13-18 kDa
• GenBank-Zugangsnummer: BC004308
• Gene symbol: PSMG3
• Gene ID (NCBI): 84262
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMG3, also named as C7orf48 and PAC3, is a chaperone protein which promotes assembly of the 20S proteasome. It may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes.