PSME3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSME3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14907-1-AP

Synonyme

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

---

Galerie der Validierungsdaten

• 

  WB analysis using 14907-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14907-1-AP (PSME3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14907-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14907-1-AP (PSME3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14907-1-AP

  WB result of PSME3 antibody (14907-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME3 transfected HepG2 cells.

• 

  WB analysis of mouse spleen using 14907-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14907-1-AP (PSME3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 14907-1-AP

  IP Result of anti-PSME3 (IP:14907-1-AP, 3ug; Detection:14907-1-AP 1:500) with COLO 320 cells lysate 2000ug.

• 

  IHC staining of human kidney using 14907-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14907-1-AP (PSME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14907-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14907-1-AP (PSME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14907-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSME3 antibody 14907-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Caco-2-Zellen, HCT 116-Zellen, HepG2-Zellen, Mausmilzgewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14907-1-AP bindet in WB, IP, IHC, IF, ELISA PSME3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSME3 fusion protein Ag6702
• Vollständiger Name: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001423
• Gene symbol: PSME3
• Gene ID (NCBI): 10197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Lett; Proteasome activator subunit 3 promotes pancreatic cancer growth via c-Myc-glycolysis signaling axis.; Authors - Jiefang Guo
human	IHC	EBioMedicine; Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.; Authors - Zhen Yu ; KD Validated
human	WB	Retrovirology; Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.; Authors - Doueiri Rami R
human	WB,IHC	PeerJ; REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma.; Authors - Zhiqiang Yin ; KD Validated
	IF	Nat Aging; Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes; Authors - Hyun Ju Lee