PSMD7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMD7 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16034-1-AP

Synonyme

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

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Galerie der Validierungsdaten

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  WB analysis using 16034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16034-1-AP

  WB result of PSMD7 antibody (16034-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells.

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  IP experiment of K-562 using 16034-1-AP

  IP Result of anti-PSMD7 (IP:16034-1-AP, 3ug; Detection:16034-1-AP 1:500) with K-562 cells lysate 2400ug.

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  IHC staining of human colon cancer using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16034-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMD7 antibody 16034-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 16034-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PSMD7 antibody (16034-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Mauslebergewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

16034-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA PSMD7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD7 fusion protein Ag8905
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
• Berechnetes Molekulargewicht: 324 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC012606
• Gene symbol: PSMD7
• Gene ID (NCBI): 5713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

Publikationen

Species	Application	Title
human	WB,CoIP	Int J Biol Sci; Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.; Authors - Jianjiang Wang ; KD Validated
human	WB,IF,CoIP	Cell Signal; Deubiquitinating enzyme PSMD7 promotes bladder cancer development: Involvement of RAB1A stabilization; Authors - Jun Wang