PSMD5 Monoklonaler Antikörper

PSMD5 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5F7

Kat-Nr. : 67682-1-Ig

Synonyme

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

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Galerie der Validierungsdaten

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  WB analysis of pig liver using 67682-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67682-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 67682-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit liver using 67682-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67682-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 67682-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, HCT 116-Zellen, K-562-Zellen, Kaninchenlebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67682-1-Ig bindet in WB, IHC, IF, ELISA PSMD5 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMD5 fusion protein Ag19298
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC014478
• Gene symbol: PSMD5
• Gene ID (NCBI): 5711
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.