PSMD2 Polyklonaler Antikörper

PSMD2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 14748-1-AP

Synonyme

26S proteasome subunit p97, P97, Protein 55.11, PSMD2, Rpn1, S2, TRAP2

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Galerie der Validierungsdaten

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  WB analysis using 14748-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14748-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14748-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14748-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14748-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14748-1-AP

  IP Result of anti-PSMD2 (IP:14748-1-AP, 4ug; Detection:14748-1-AP 1:1000) with K-562 cells lysate 3320ug.

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  IHC staining of human breast cancer using 14748-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14748-1-AP (PSMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14748-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14748-1-AP (PSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A431-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Herzgewebe, K-562-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, PC-3-Zellen, Rattenherzgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

14748-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA PSMD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD2 fusion protein Ag6484
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC002368
• Gene symbol: PSMD2
• Gene ID (NCBI): 5708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor type 1 receptor-associated protein 2 (TRAP2), encoded by PSMD2 gene, is a non-ATPase regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. TRAP2 may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor.

Publikationen

Species	Application	Title
human	WB	Cell; Cancer vulnerabilities unveiled by genomic loss.; Authors - Nijhawan Deepak D
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
human	WB	Cells; The Identification of Nuclear FMRP Isoform Iso6 Partners; Authors - Nassim Ledoux
human	WB,CoIP	Cell Commun Signal; NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma.; Authors - Hong-Zhong Zhou
human	WB	BMC Mol Biol; PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism.; Authors - Yanjie Tan
human,mouse	WB,CoIP	J Exp Clin Cancer Res; AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma.; Authors - Chunyan Gu