PSMC6 Polyklonaler Antikörper

PSMC6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15839-1-AP

Synonyme

CADP44, p42, P44, Proteasome subunit p42, PSMC6, Rpt4, SUG2

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Galerie der Validierungsdaten

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  WB analysis using 15839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15839-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15839-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15839-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15839-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, humanes Hodengewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

15839-1-AP bindet in WB, IHC, ELISA PSMC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMC6 fusion protein Ag8589
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, ATPase, 6
• Berechnetes Molekulargewicht: 389 aa, 44 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC005390
• Gene symbol: PSMC6
• Gene ID (NCBI): 5706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMC6(proteasome (prosome, macropain) 26S subunit, ATPase, 6) is also named as CADP44, MGC12520, P44, SUG2, p42 and belongs to the AAA ATPase family. It is a component of the 26S protease, which is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. The gene encodes a 44 kDa PSMC6.

Publikationen

Species	Application	Title
mouse	WB	J Biomed Sci; Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.; Authors - Yi-Chao Lee
mouse	IHC	FASEB J; Glucose-induced decrease of cystathionine β-synthase mediates renal injuries.; Authors - Yanting Yu
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
mouse,rat	WB	Acta Pharmacol Sin; Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition; Authors - Qian Wu