PSMC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMC2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14905-1-AP

Synonyme

MSS1, Nbla10058, Protein MSS1, PSMC2, S7

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Galerie der Validierungsdaten

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  WB analysis using 14905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14905-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 14905-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14905-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 14905-1-AP

  IP Result of anti-PSMC2 (IP:14905-1-AP, 4ug; Detection:14905-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Nebennierengewebe, Mausmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below

Produktinformation

14905-1-AP bindet in WB, IP, IHC, ELISA PSMC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMC2 fusion protein Ag6700
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, ATPase, 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC002589
• Gene symbol: PSMC2
• Gene ID (NCBI): 5701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMC2 is also named as MSS1 and belongs to the AAA ATPase family. PSMC2 is 1 of 6 putative ATPases contained within the regulatory complex. PSMC2 is first identified as a possible cellular factor that cooperates with the human immunodeficiency virus-1 (HIV-1) protein Tat, a potent activator of virus gene expression. The PSMC2 gene product works in association with the small subunit of mitoribosomes and seems to play some part in mitochondrial translation(PMID:8392589).

Publikationen

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
mouse	IHC,WB	Calcif Tissue Int; Silencing Proteasome 26S Subunit ATPase 2 (PSMC2) Protects the Osteogenic Differentiation In Vitro and Osteogenesis In Vivo.; Authors - Bin Zhou ; KD Validated
human	IHC	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su
human	IHC	Pharmgenomics Pers Med; PSMC2 is a Novel Prognostic Biomarker and Predicts Immunotherapeutic Responses: From Pancreatic Cancer to Pan-Cancer; Authors - Wei Huang