PSMB5 Polyklonaler Antikörper

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• KD/KO Validated

PSMB5 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 19178-1-AP

Synonyme

20S proteasome, LMPX, Macropain epsilon chain, MB1, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit beta type 5, Proteasome subunit MB1, Proteasome subunit X, PSMB5

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Galerie der Validierungsdaten

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  WB analysis using 19178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19178-1-AP

  WB result of PSMB5 antibody (19178-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.

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  IHC staining of human breast cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19178-1-AP (PSMB5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

19178-1-AP bindet in WB, IHC, IF, ELISA PSMB5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMB5 fusion protein Ag5828
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 5
• Berechnetes Molekulargewicht: 263 aa, 28 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC057840
• Gene symbol: PSMB5
• Gene ID (NCBI): 5693
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell; Cancer vulnerabilities unveiled by genomic loss.; Authors - Nijhawan Deepak D
human	WB	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin
human	IF,WB	Cancer Lett; Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma; Authors - Hongmei Luo
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
human	WB	J Cancer; Cytoplasm-localized SIRT1 downregulation attenuates apoptosis and cell cycle arrest in cisplatin-resistant lung cancer A549 cells.; Authors - Hyeran Yu
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie