PSMB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMB2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15154-1-AP

Synonyme

20S proteasome, HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

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Galerie der Validierungsdaten

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  WB analysis using 15154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15154-1-AP

  WB result of PSMB2 antibody (15154-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells.

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  WB analysis of HEK-293 using 15154-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15154-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15154-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15154-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 15154-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 15154-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15154-1-AP

  Immunofluorescent analysis of HepG2 cells using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

15154-1-AP bindet in WB, IHC, IF, ELISA PSMB2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMB2 fusion protein Ag7198
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 2
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000268
• Gene symbol: PSMB2
• Gene ID (NCBI): 5690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB2, Proteasome subunit beta type-2, is also known as 20S proteasome subunit beta-4(PMID: 7918633). The proteasome is responsible for degradation of short lived and misfolded cytosolic and nuclear proteins in the cell and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMB2 is up-regulated in ovarian cancer cell lines. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD (vitamin A depletion) brain regions (PMID: 21190828). Knockdown of PSMB2 suppresses hepatocellular carcinoma and gastric cancer cell proliferation and invasion (PMID: 29780166, PMID: 36110152).

Publikationen

Species	Application	Title
human	WB	Oncogene; NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.; Authors - Sheng Tan ; KD Validated
human	WB	Mol Cancer Res; Proteasome subunits differentially control Myeloma cell viability and proteasome inhibitor sensitivity.; Authors - Chang-Xin Shi
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
rat	WB	Metab Brain Dis; Molecular alteration of the proteasome contributes to AD-like pathology in the brain of HFD-STZ diabetic rats; Authors - Han Gao
rat	WB	Cell Mol Neurobiol; Inhibition of the Proteasome Regulator PA28 Aggravates Oxidized Protein Overload in the Diabetic Rat Brain; Authors - Dong-Gui Wu