PSMA7 Polyklonaler Antikörper

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• KD/KO Validated

PSMA7 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 15219-1-AP

Synonyme

20S proteasome, C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15219-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15219-1-AP (PSMA7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15219-1-AP

  WB result of PSMA7 antibody (15219-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells.

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  IP experiment of mouse brain using 15219-1-AP

  IP Result of anti-PSMA7 (IP:15219-1-AP, 4ug; Detection:15219-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15219-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15219-1-AP (PSMA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15219-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PSMA7 antibody (15219-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:15000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below

Produktinformation

15219-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PSMA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA7 fusion protein Ag7389
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC004427
• Gene symbol: PSMA7
• Gene ID (NCBI): 5688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

Publikationen

Species	Application	Title
mouse	WB	Brain; Raising cGMP restores proteasome function and myelination in mice with a proteotoxic neuropathy.; Authors - Jordan J S VerPlank
mouse	WB	Glia; Impairment of protein degradation and proteasome function in hereditary neuropathies.; Authors - Jordan J S VerPlank
human	WB	Mol Cell Proteomics; Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.; Authors - Tatjana Goebel
human	WB	Biochim Biophys Acta Mol Basis Dis; Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.; Authors - Diego Sbardella
human	WB	Cell Physiol Biochem; PSMA7 Directly Interacts with NOD1 and Regulates its Function.; Authors - Yang Liuzhong L
rat	WB	Exp Cell Res; The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models.; Authors - Han Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000