PSMA/GCPII Monoklonaler Antikörper

PSMA/GCPII Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3G4E12

Kat-Nr. : CL594-66678

Synonyme

FGCP, Folate hydrolase 1, FOLH, FOLH1, GCP2, GCPII, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, mGCP, NAALAD1, NAALAdase, NAALADase I, PSM, PSMA, PSMA/GCPII

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Galerie der Validierungsdaten

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  IF Staining of human prostate cancer using CL594-66678

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

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  IF Staining of PC-3 using CL594-66678

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

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  FC experiment of LNCaP using CL594-66678

  1X10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMA/GCPII (CL594-66678, Clone:3G4E12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: PC-3-Zellen, humanes Prostatakarzinomgewebe
• Erfolgreiche Detektion in FC: LNCaP-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66678 bindet in IF, FC (Intra) PSMA/GCPII und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMA/GCPII fusion protein Ag16594
• Vollständiger Name: folate hydrolase (prostate-specific membrane antigen) 1
• Berechnetes Molekulargewicht: 719 aa, 81 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC025672
• Gene symbol: FOLH1
• Gene ID (NCBI): 2346
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.