PSAP Polyklonaler Antikörper

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• KD/KO Validated

PSAP Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18396-1-AP

Synonyme

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 18396-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18396-1-AP

  WB result of PSAP antibody (18396-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

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  WB analysis of human liver using 18396-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18396-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18396-1-AP (PSAP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 18396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18396-1-AP (PSAP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18396-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18396-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18396-1-AP bindet in WB, IHC, IF, ELISA PSAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSAP fusion protein Ag13263
• Vollständiger Name: prosaposin
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 70 kDa
• GenBank-Zugangsnummer: BC001503
• Gene symbol: PSAP
• Gene ID (NCBI): 5660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PSAP gene encodes prosaposin, a precursor of fourl small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin A.