PRSS8 Polyklonaler Antikörper

PRSS8 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15527-1-AP

Synonyme

CAP1, PROSTASIN, protease, serine, 8, PRSS8, Serine protease 8

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Galerie der Validierungsdaten

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  WB analysis of human colon using 15527-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15527-1-AP (PRSS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15527-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15527-1-AP (PRSS8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15527-1-AP (PRSS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15527-1-AP (PRSS8 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15527-1-AP (PRSS8 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 5 publications below

Produktinformation

15527-1-AP bindet in WB, IHC, ELISA PRSS8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRSS8 fusion protein Ag7234
• Vollständiger Name: protease, serine, 8
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC001462
• Gene symbol: PRSS8
• Gene ID (NCBI): 5652
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protease serine S1 family member 8 (PRSS8), a membrane-anchored serine protease, has been reported to be involved in the development of several human cancers. PRSS8 protein level was highly expressed in well differentiated cancer cell and was lower or absent in poorly differentiated cancer cell. Significantly higher levels of prostasin were found in early stage OVC serum samples compared to benign ovarian and normal donor samples. Overexpression of PRSS8 mRNA and high levels of prostasin in multiple subtypes of early stage ovarian tumors may provide clinical biomarkers for early detection of OVC. (PMID: 27036110, PMID: 27050145)

Publikationen

Species	Application	Title
mouse	WB,IHC	Acta Physiol (Oxf); Zymogen-locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice.; Authors - Daniel Essigke
mouse	WB	Br J Pharmacol; PMID: 31222723; Authors - Ming-Ming Wu
human	WB,IHC	J Hypertens; Prostasin and matriptase (ST14) in placenta from preeclamptic and healthy pregnant women.; Authors - Britta Frederiksen-Møller
human	WB,IHC	Acta Physiol (Oxf); Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium.; Authors - Steensgaard M M
human	WB	Pflugers Arch; The epithelial Na+ channel α- and γ-subunits are cleaved at predicted furin-cleavage sites, glycosylated and membrane associated in human kidney.; Authors - Rikke Zachar
human	WB	Nephrol Dial Transplant; PMID: 25609736; Authors - Henrik Andersen