PRSS27 Polyklonaler Antikörper

PRSS27 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19515-1-AP

Synonyme

CAPH2, Marapsin, MPN, Pancreasin, protease, serine 27, PRSS27, Serine protease 27

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Galerie der Validierungsdaten

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  WB analysis of SW 1990 using 19515-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 19515-1-AP (PRSS27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 19515-1-AP

  IP Result of anti-PRSS27 (IP:19515-1-AP, 4ug; Detection:19515-1-AP 1:300) with SW 1990 cells lysate 2960ug.

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  IHC staining of human pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human oesophagus cancer using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SW 1990-Zellen
• Erfolgreiche IP: SW 1990-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Ösophaguskarzinomgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19515-1-AP bindet in WB, IP, IHC, ELISA PRSS27 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protease, serine 27
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: NM_031948
• Gene symbol: PRSS27
• Gene ID (NCBI): 83886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRSS27, also named as CAPH2, MPN, belongs to the peptidase S1 family. The trypsin-like serine protease marapsin (Prss27) is a member of the large protease gene cluster at human chromosome 16p13.3. The Prss27 gene is expressed by a variety of human, mouse, and opossum tissues. In humans and mice, it is especially abundant in stratified squamous epithelia of cornea, larynx, esophagus, and cervix and is induced in wounded and psoriatic skin (PMID: 23447538). Prss27 expression was restricted to tissues containing stratified squamous epithelia and was absent or only weakly expressed in all other tissues, including the pancreas.