PRSS2 Polyklonaler Antikörper

PRSS2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15005-1-AP

Synonyme

Anionic trypsinogen, PRSS2, Serine protease 2, TRY2, TRY8, TRYP2, Trypsin 2, Trypsin II

---

Galerie der Validierungsdaten

• 

  WB analysis of Raji using 15005-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15005-1-AP (PRSS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15005-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15005-1-AP (PRSS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15005-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15005-1-AP (PRSS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 15005-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15005-1-AP (PRSS2 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human small intestine using 15005-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15005-1-AP (PRSS2 Antibody) at dilution of 1:400 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HepG2-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

15005-1-AP bindet in WB, IHC, ELISA PRSS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRSS2 fusion protein Ag6461
• Vollständiger Name: protease, serine, 2 (trypsin 2)
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa, 35 kDa
• GenBank-Zugangsnummer: BC030260
• Gene symbol: PRSS2
• Gene ID (NCBI): 5645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human pancreas produces three isoforms of trypsinogen encoded by separate genes, the PRSS1 (protease, serine 1), PRSS2, and PRSS3 genes. PRSS2 is also named as TRY2(trypsin-2), TRYP2. In the ileum, it may be involved in defensin processing, including DEFA5. The preproprotein comprises 247 amino acids, including a 15-amino acid signal peptide and an 8-amino acid activation peptide.

Publikationen

Species	Application	Title
mouse	WB	Biol Open; Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.; Authors - Timothy L Hostelley
human,mouse	WB,IHC	Tissue Cell; PRSS2 overexpression relates to poor prognosis and promotes proliferation, migration and invasion in gastric cancer; Authors - Yu Chen ; KD Validated
mouse	IHC	Biochem Biophys Res Commun; Epigenome-wide association analysis of pancreatic exocrine cells from high-fat- and normal diet-fed mice and its potential use for understanding the oncogenesis of human pancreatic cancer; Authors - Tomoyuki Araki
human	WB	Acta Histochem; PRSS2 regulates EMT and metastasis via MMP-9 in gastric cancer; Authors - Fei Wang ; KD Validated