PRR14 Polyklonaler Antikörper

PRR14 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22819-1-AP

Synonyme

proline rich 14, Proline rich protein 14, PRR14

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Galerie der Validierungsdaten

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  WB analysis using 22819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22819-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 22819-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HL-60-Zellen, MCF-7-Zellen, SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

22819-1-AP bindet in WB, IF, ELISA PRR14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRR14 fusion protein Ag14119
• Vollständiger Name: proline rich 14
• Berechnetes Molekulargewicht: 585 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64-70 kDa
• GenBank-Zugangsnummer: BC000119
• Gene symbol: PRR14
• Gene ID (NCBI): 78994
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRR14 (proline-rich protein 14) is a 585-amino acid protein with a calculated molecular weight of 64 kDa. This protein functions to tether heterochromatin to the nuclear periphery during interphase, through associations with heterochromatin protein 1 (HP1) and the nuclear lamina. After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. (PMID: 24209742; 24637393)

Publikationen

Species	Application	Title
mouse	WB,IF	Nucleus; PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina; Authors - Anna A Kiseleva ; KD Validated