PRPF6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRPF6 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 23929-1-AP

Synonyme

ANT 1, C20orf14, hPrp6, Pre mRNA processing factor 6, PRP6 homolog, PRPF6, TOM, U5 102 kDa protein, U5 102K

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23929-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23929-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23929-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23929-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23929-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23929-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23929-1-AP

  IP Result of anti-PRPF6 (IP:23929-1-AP, 4ug; Detection:23929-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human testis using 23929-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23929-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

23929-1-AP bindet in WB, IP, IHC, IF, ELISA PRPF6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF6 fusion protein Ag13817
• Vollständiger Name: PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 941 aa, 107 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC001666
• Gene symbol: PRPF6
• Gene ID (NCBI): 24148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

Publikationen

Species	Application	Title
human	WB	Hum Genet; A CRISPR and high-content imaging assay compliant with ACMG/AMP guidelines for clinical variant interpretation in ciliopathies.; Authors - Liliya Nazlamova
mouse	WB,IF,IHC	Cancer Sci; Splicing factor PRPF6 upregulates oncogenic androgen receptor signaling pathway in hepatocellular carcinoma.; Authors - Huijuan Song ; KD Validated
human	WB	Int J Biol Sci; PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.; Authors - Wei Liu ; KD Validated
human,mouse	IF,WB	Front Genet; Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations; Authors - Liliya Nazlamova