PRMT5 Polyklonaler Antikörper

PRMT5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27109-1-AP

Synonyme

72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs

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Galerie der Validierungsdaten

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  WB analysis using 27109-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 27109-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27109-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 27109-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27109-1-AP bindet in WB, ELISA PRMT5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRMT5 fusion protein Ag25896
• Vollständiger Name: protein arginine methyltransferase 5
• Berechnetes Molekulargewicht: 637 aa, 73 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC025979
• Gene symbol: PRMT5
• Gene ID (NCBI): 10419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.