PRM1 Polyklonaler Antikörper

PRM1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 15697-1-AP

Synonyme

P1, PRM1

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Galerie der Validierungsdaten

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  WB analysis using 15697-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15697-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 15697-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 15697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15697-1-AP (PRM1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

15697-1-AP bindet in WB, IHC, ELISA PRM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRM1 fusion protein Ag8323
• Vollständiger Name: PRM1
• Berechnetes Molekulargewicht: 51 aa, 6 kDa
• Beobachtetes Molekulargewicht: 13-20 kDa
• GenBank-Zugangsnummer: BC003673
• Gene symbol: PRM1
• Gene ID (NCBI): 5619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)

Publikationen

Species	Application	Title
mouse	WB	Environ Pollut; Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.; Authors - Jianhui Liu
mouse	WB	Int J Mol Sci; BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.; Authors - Chao Huang
mouse	WB	Theriogenology; Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5; Authors - Yunxiang Li
mouse	WB	Reprod Sci; Sertoli Cell-Conditioned Medium Induces Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model; Authors - Nasrin Khanmohammadi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chongil (Verified Customer) (08-19-2021)

Prm1 antibody may detect a wrong protein. Prm1 is detected in elongatid spermatids and testicular sperm heads. However, this antibody detected in spermatocytes, round spermatids, and sperm tails but not in sperm heads.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse testis section

PRM1 Antibody Immunofluorescence validation (1:300 dilution) in Mouse testis section (Cat no:15697-1-AP)