DNA-PKcs Polyklonaler Antikörper

DNA-PKcs Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19983-1-AP

Synonyme

DNA PK catalytic subunit, DNAPK, DNA-PKcs, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, MCF-7-Zellen
Erfolgreiche IPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19983-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA DNA-PKcs und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name protein kinase, DNA-activated, catalytic polypeptide
Berechnetes Molekulargewicht 469 kDa
Beobachtetes Molekulargewicht 350-460 kDa
GenBank-ZugangsnummerNM_006904
Gene symbol PRKDC
Gene ID (NCBI) 5591
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.

Protokolle

Produktspezifische Protokolle
WB protocol for DNA-PKcs antibody 19983-1-APProtokoll herunterladen
IP protocol for DNA-PKcs antibody 19983-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Science

IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV.

Authors - Kathryn M Monroe
humanWB

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
humanIF, WB

J Cell Biol

DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.

Authors - Shuyun Ma
humanWB

Int J Biol Macromol

Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation

Authors - Hong-Yu Tao
humanWB,CoIP

Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer

Authors - Chen Zhou
humanWB

Cell Biol Toxicol

Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway.

Authors - Ruixue Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHAO (Verified Customer) (03-31-2022)

Strong correct bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart