PKC Delta Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PKC Delta Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (2)

Kat-Nr. : 14188-1-AP

Synonyme

MAY1, PKC delta, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

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Galerie der Validierungsdaten

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  WB analysis using 14188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14188-1-AP (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14188-1-AP

  IP result of anti-PKC Delta(IP:14188-1-AP, 4ug; Detection:14188-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

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  IF Staining of HeLa using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of Saos-2 using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C6-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF: Saos-2 cells, A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14188-1-AP bindet in WB, IP, IHC, IF, ELISA PKC Delta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Delta fusion protein Ag5473
• Vollständiger Name: protein kinase C, delta
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC043350
• Gene symbol: PRKCD
• Gene ID (NCBI): 5580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Discovery of a signaling feedback circuit that defines interferon responses in myeloproliferative neoplasms.; Authors - Diana Saleiro ; KD Validated
mouse	WB	JCI Insight; Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.; Authors - Hongyi Zhou
rat	WB	Mol Cancer Res; PKCð regulates chromatin remodeling and DNA repair through SIRT6; Authors - Trisiani Affandi
human	WB	J Biol Chem; Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double strand break repair.; Authors - Trisiani Affandi
human,mouse	WB,IHC	Food Funct; Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.; Authors - Lingli Sun
human	WB	Chem Biol Interact; ZCL-082, a boron-containing compound, induces apoptosis of non-Hodgkin's lymphoma via targeting p90 ribosomal S6 kinase 1/NF-κB signaling pathway.; Authors - Chunmin Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wassim (Verified Customer) (04-23-2019)

This antibody picks a band ~76 KDa in mouse kidney tubular cells lysed in RIPA buffer.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Kidney Epithelial Cells

PKC Delta Antibody Western Blot, validation (1:3000 dilution) in Mouse Kidney Epithelial Cells (Cat no:14188-1-AP)

FH

Angela (Verified Customer) (12-17-2018)

Lanes are as follows: 1 SCR control2 pkcd shrna13 pkcd shrna24 pkcd shrna35 50uM etoposide 18hr6 untreated control

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Salivary cell line

PKC Delta Antibody Western Blot, validation (1:1000 dilution) in Salivary cell line (Cat no:14188-1-AP)