PRKAR1A Polyklonaler Antikörper

PRKAR1A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 20358-1-AP

Synonyme

CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, PRKAR1A, Tissue specific extinguisher 1, TSE1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 20358-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 20358-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20358-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20358-1-AP (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20358-1-AP bindet in WB, IHC, IF, ELISA PRKAR1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKAR1A fusion protein Ag14216
• Vollständiger Name: protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
• Berechnetes Molekulargewicht: 381 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC036285
• Gene symbol: PRKAR1A
• Gene ID (NCBI): 5573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner