PRKACB Polyklonaler Antikörper

PRKACB Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 55382-1-AP

Synonyme

PKA C beta, PKA C-beta-specific, PKACB, PRKACB

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 55382-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55382-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55382-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 55382-1-AP (PKA C-beta-specifc antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 55382-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 55382-1-AP (PKA C-beta-specifc antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 3 publications below

Produktinformation

55382-1-AP bindet in WB, IHC, ELISA PRKACB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase, cAMP-dependent, catalytic, beta
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 36-55 kDa
• GenBank-Zugangsnummer: NM_002731
• Gene symbol: PRKACB
• Gene ID (NCBI): 5567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. This antibody is specific to PRKACB.

Publikationen

Species	Application	Title
human,mouse	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
mouse	WB	Int J Mol Sci; Per1/Per2 Disruption Reduces Testosterone Synthesis and Impairs Fertility in Elderly Male Mice.; Authors - Qinrui Liu
human	WB	Oncogenesis; GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.; Authors - Chenggang Zhao
human	WB	Pharmacol Res; Hypericin attenuates nonalcoholic fatty liver disease and abnormal lipid metabolism via the PKA-mediated AMPK signaling pathway in vitro and in vivo.; Authors - Chen Liang
human	IHC	Am J Cancer Res; Different lipid metabolic profiles and their associated genes in sessile serrated adenoma or polyps compared to hyperplastic polyps.; Authors - Chaotao Tang
human	WB	Viruses; ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells; Authors - Beibei Zhang