PKA C-alpha specific Polyklonaler Antikörper

PKA C-alpha specific Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 55388-1-AP

Synonyme

PKA C alpha, PKACA, PRKACA

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Galerie der Validierungsdaten

• 

  WB analysis of human testis using 55388-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 55388-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 55388-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 55388-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55388-1-AP

  IP Result of anti-PKA C-alpha speicifc (IP:55388-1-AP, 4ug; Detection:55388-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human colon cancer using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human colon cancer using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human testis using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human testis using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, humanes Lebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55388-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA PKA C-alpha specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase, cAMP-dependent, catalytic, alpha
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: NM_002730
• Gene symbol: PRKACA
• Gene ID (NCBI): 5566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.

Publikationen

Species	Application	Title
human	WB,IF,IP	Sci Total Environ; Prolonged exposure of environmental concentration benzo[a]pyrene promoted cancer stemness through AhR/PKA/SOX2 dependent pathway in small cell lung cancer; Authors - Heng Ni
		Oncotarget; Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland.; Authors - Laura C Hernández-Ramírez
mouse	WB	Front Pharmacol; An Integrative Pharmacology-Based Strategy to Uncover the Mechanism of Xiong-Pi-Fang in Treating Coronary Heart Disease with Depression.; Authors - Lihong Zhang
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
human	WB	Proteomics; Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein Upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.; Authors - Hwee Tong Tan
		CNS Neurosci Ther; Increased Expression of Transient Receptor Potential Vanilloid 4 in Cortical Lesions of Patients with Focal Cortical Dysplasia.; Authors - Xin Chen