peroxiredoxin 2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

peroxiredoxin 2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20569-1-AP

Synonyme

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 20569-1-AP

  WB result of peroxiredoxin 2 antibody (20569-1-AP, 1:5000) with si-Control and si-peroxiredoxin 2 transfected HeLa cells.

• 

  WB analysis of HeLa using 20569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20569-1-AP

  Immunofluorescent analysis of HepG2 cells, using PRDX2 antibody 20569-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20569-1-AP bindet in WB, IF, ELISA peroxiredoxin 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: peroxiredoxin 2
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_005809
• Gene symbol: PRDX2
• Gene ID (NCBI): 7001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX2, also named as NKEFB, TDPX1, TSA and PRP, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. PRDX2 may play an important role in eliminating peroxides generated during metabolism. It might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The antibody is specific to PRDX2.