PRC1 Polyklonaler Antikörper

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• KD/KO Validated

PRC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 15617-1-AP

Synonyme

ASE1, PRC1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 15617-1-AP

  WB result of PRC1 antibody (15617-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

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  WB analysis using 15617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15617-1-AP (PRC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15617-1-AP

  IP Result of anti-PRC1 (IP:15617-1-AP, 4ug; Detection:15617-1-AP 1:300) with HEK-293 cells lysate 1480ug.

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  IHC staining of human breast cancer using 15617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15617-1-AP (PRC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15617-1-AP (PRC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (15617-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Raji-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Produktinformation

15617-1-AP bindet in WB, IP, IHC, IF, ELISA PRC1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRC1 fusion protein Ag8028
• Vollständiger Name: protein regulator of cytokinesis 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC003138
• Gene symbol: PRC1
• Gene ID (NCBI): 9055
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

Publikationen

Species	Application	Title
human	WB,IHC	Nat Commun; Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer.; Authors - Jing Li
mouse	IF	Dev Cell; Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse.; Authors - Aleksandar I Mihajlović
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
mouse	WB	Development; Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.; Authors - Mansour Aboelenain
human	WB,IHC	Front Cell Dev Biol; PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.; Authors - Shixin Liao