PR Polyklonaler Antikörper

PR Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 25871-1-AP

Synonyme

11, NR3C3, PGR, PR, progesterone receptor

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Galerie der Validierungsdaten

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  WB analysis of T-47D using 25871-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 25871-1-AP (PR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of T-47D using 25871-1-AP

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using PR antibody (25871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: T-47D-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 11 publications below
• IF: See 1 publications below

Produktinformation

25871-1-AP bindet in WB, IHC, IF, ELISA PR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PR fusion protein Ag22986
• Vollständiger Name: progesterone receptor
• Berechnetes Molekulargewicht: 933 aa, 99 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 110-115 kDa
• GenBank-Zugangsnummer: NM_000926
• Gene symbol: PGR
• Gene ID (NCBI): 5241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance.; Authors - Yalan Deng
human	IF,WB	Nucleic Acids Res; ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD; Authors - Jumin Park
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku
goat	WB,IHC	Int J Mol Sci; The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats; Authors - Zhen Gao
rat	WB,IHC	Front Endocrinol (Lausanne); Effect of acupuncture on the opening time of implantation window and endometrial receptivity in controlled ovarian hyperstimulation rats during peri-implantation period; Authors - Runan Hu
human	IHC	Front Oncol; Pan-Cancer Prognostic Role and Targeting Potential of the Estrogen-Progesterone Axis.; Authors - Yu-Ting Shen