PPT1 Polyklonaler Antikörper

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• KD/KO Validated

PPT1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29653-1-AP

Synonyme

CLN1, EC:3.1.2.22, INCL, Palmitoyl-protein hydrolase 1, Palmitoyl-protein thioesterase 1

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  WB analysis using 29653-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29653-1-AP (PPT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 29653-1-AP

  WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

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  IHC staining of human kidney using 29653-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29653-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 10x lens). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 29653-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, Maushodengewebe, Rattenhodengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

29653-1-AP bindet in WB, IHC, IF-P, ELISA PPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPT1 fusion protein Ag31240
• Vollständiger Name: palmitoyl-protein thioesterase 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 32-34 kDa
• GenBank-Zugangsnummer: BC008426
• Gene symbol: PPT1
• Gene ID (NCBI): 5538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv ; KD Validated
human	WB	EMBO Rep; Palmitoylation by ZDHHC4 inhibits TRPV1-mediated nociception; Authors - Youjing Zhang