PPP2R2B/A/C/D Polyklonaler Antikörper

PPP2R2B/A/C/D Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 29237-1-AP

Synonyme

B55 BETA, B55β, FLJ95686, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform beta, PP2A subunit B isoform R2 beta, PP2AB BETA, PP2APR55 BETA, PPP2R2B, PPP2R2B/A/C/D, PR2AB BETA, PR2AB55 BETA, PR2APR55 BETA, PR52B, PR55 BETA, SCA12

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Galerie der Validierungsdaten

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  WB analysis using 29237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29237-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29237-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29237-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:400-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29237-1-AP bindet in WB, IHC, ELISA PPP2R2B/A/C/D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R2B/A/C/D fusion protein Ag30757
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Berechnetes Molekulargewicht: 443 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC031790
• Gene symbol: PPP2R2B
• Gene ID (NCBI): 5521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PPP2R2B gene encodes a deduced 443 amino acid protein of approximately 52 kDa, which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. The antibody cross-reacts with PPP2R2A, PPP2R2C, and PPP2R2D due to high immunogen homology between PPP2R2B and PPP2R2A, PPP2R2C, and PPP2R2D.

Publikationen

Species	Application	Title
mouse	WB	Biochim Biophys Acta Mol Cell Res; Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport; Authors - G F Sengül