Neurabin 2 Polyklonaler Antikörper

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• KD/KO Validated

Neurabin 2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55129-1-AP

Synonyme

Neurabin 2, Neurabin II, PPP1R6, PPP1R9, PPP1R9B, SPINO, Spinophilin, Spn

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55129-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55129-1-AP (Neurabin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55129-1-AP

  WB result of PPP1R9B antibody (55129-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neurabin 2 transfected A549 cells.

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  IP experiment of mouse brain using 55129-1-AP

  IP Result of anti-Neurabin 2 (IP:55129-1-AP, 4ug; Detection:55129-1-AP 1:300) with mouse brain tissue lysate 6000ug.

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  IHC staining of human brain using 55129-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55129-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

55129-1-AP bindet in WB, IP, IHC, ELISA Neurabin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_032595
• Gene symbol: PPP1R9B
• Gene ID (NCBI): 84687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).

Publikationen

Species	Application	Title
human	WB,IP	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
	WB	Toxicology; Changes in memory and synaptic plasticity induced in male rats after maternal exposure to Bisphenol A.; Authors - Chong Wang
mouse	WB	Genes Dis; Hippocampal overexpression of TREM2 ameliorates high fat diet induced cognitive impairment and modulates phenotypic polarization of the microglia.; Authors - Min Wu