iASPP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

iASPP Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 18590-1-AP

Synonyme

IASPP, Inhibitor of ASPP protein, NFkB interacting protein 1, NKIP1, PPP1R13B like protein, PPP1R13BL, PPP1R13L, Protein iASPP, RAI, RelA associated inhibitor

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Galerie der Validierungsdaten

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  WB analysis using 18590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Apoptosised HeLa using 18590-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18590-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 18590-1-AP

  IP Result of anti-iASPP (IP:18590-1-AP, 4ug; Detection:18590-1-AP 1:1000) with PC-3 cells lysate 1040ug.

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  IHC staining of human breast cancer using 18590-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18590-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 18590-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PPP1R13L antibody 18590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Apoptotisierte HeLa-Zellen, C6-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

18590-1-AP bindet in WB, IP, IHC, IF, ELISA iASPP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: iASPP fusion protein Ag13273
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC064913
• Gene symbol: PPP1R13L
• Gene ID (NCBI): 10848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53- mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression. This antibody could both recognize unphosphorylated and phosphorylated iASPP.

Publikationen

Species	Application	Title
human	WB,IP	J Cell Biol; iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.; Authors - Aurélie Mangon ; KD Validated
human	WB,IP,IF	Cell Death Dis; iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.; Authors - Kun Gao ; KD Validated
mouse	WB	J Biochem Mol Toxicol; Upregulation of iASPP ameliorates hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes by upregulating Nrf2 signaling.; Authors - Baobao Bai ; KD Validated
mouse	WB	Acta Biochim Biophys Sin (Shanghai); iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis.; Authors - Timur Yagudin