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PPM1K Polyklonaler Antikörper
PPM1K Polyklonal Antikörper für IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14573-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | humanes Hirngewebe, humanes Herzgewebe |
| Erfolgreiche Detektion in IHC | humanes Gliomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunhistochemie (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Produktinformation
14573-1-AP bindet in WB, IHC, IF, ELISA PPM1K und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | PPM1K fusion protein Ag5660 |
| Vollständiger Name | protein phosphatase 1K (PP2C domain containing) |
| Berechnetes Molekulargewicht | 41 kDa |
| Beobachtetes Molekulargewicht | 30-40 kDa |
| GenBank-Zugangsnummer | BC037552 |
| Gene symbol | PPM1K |
| Gene ID (NCBI) | 152926 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for PPM1K antibody 14573-1-AP | Protokoll herunterladen |
| IHC protocol for PPM1K antibody 14573-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun Glucose promotes cell growth by suppressing branched-chain amino acid degradation.
| ||
Free Radic Biol Med Elevated branched-chain α-keto acids exacerbate macrophage oxidative stress and chronic inflammatory damage in type 2 diabetes mellitus. | ||
Front Pharmacol Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice. | ||
Physiol Rep Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH CHAO (Verified Customer) (01-30-2020) | Clear, sharp band as predicted size 40 kDa.
|
FH Lorenza (Verified Customer) (11-08-2019) | I have tried four different antibodies from different companies and this is the only one that works in total lysate (and not only in isolated mitochondria). All the others give an unspecific band at the same molecular weight. Works perfectly. There are some unspecific bands at higher molecular weights but the right bands is easily detectable.
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