PPIF-Specific Polyklonaler Antikörper

PPIF-Specific Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18466-1-AP

Synonyme

Cyclophilin F, Cyp D, CYP3, peptidylprolyl isomerase F, PPIase F, PPIF, Rotamase F



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, Mausherzgewebe, Rattenherzgewebe
Erfolgreiche IPMausherzgewebe
Erfolgreiche Detektion in IHChumanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:100-1:400
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18466-1-AP bindet in WB, IP, IHC, IF, ELISA PPIF-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPIF-Specific fusion protein Ag13151
Vollständiger Name peptidylprolyl isomerase F
Berechnetes Molekulargewicht 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC005020
Gene symbol PPIF
Gene ID (NCBI) 10105
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF

Protokolle

Produktspezifische Protokolle
WB protocol for PPIF-Specific antibody 18466-1-APProtokoll herunterladen
IHC protocol for PPIF-Specific antibody 18466-1-APProtokoll herunterladen
IF protocol for PPIF-Specific antibody 18466-1-APProtokoll herunterladen
IP protocol for PPIF-Specific antibody 18466-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Commun

The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury

Authors - Hakjoo Lee
humanIHC

Aging (Albany NY)

Overexpression of TICRR and PPIF confer poor prognosis in endometrial cancer identified by gene co-expression network analysis.

Authors - Linlin Yang
mouseWB

J Biol Chem

The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.

Authors - Hakjoo Lee
humanIF

PLoS One

Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM.

Authors - Wang Yun E YE
mouseWB

EMBO J

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses

Authors - Hao Liu
humanWB

Biol Pharm Bull

Cinnamtannin B-1 inhibits the progression of osteosarcoma by regulating the miR-1281/PPIF axis

Authors - Jun Jia

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Veronica (Verified Customer) (01-30-2024)

Really satisfied with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: 12% acrylammide gel Ab’: 1:2000 in 5% milk O/N

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
PPIF-Specific Antibody Western Blot validation (1:2000 dilution) in HeLa (Cat no:18466-1-AP)