PPCS Polyklonaler Antikörper

PPCS Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 18001-1-AP

Synonyme

COAB, PPC synthetase, PPCS

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18001-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18001-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

18001-1-AP bindet in WB, IHC, IF,ELISA PPCS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPCS fusion protein Ag12491
• Vollständiger Name: phosphopantothenoylcysteine synthetase
• Berechnetes Molekulargewicht: 311 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC106064
• Gene symbol: PPCS
• Gene ID (NCBI): 79717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

Publikationen

Species	Application	Title
human	IF	Int J Mol Sci; Regulation of the CoA Biosynthetic Complex Assembly in Mammalian Cells.; Authors - Jovana Baković

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jovana (Verified Customer) (09-17-2020)

The antibody worked well to detect endogenous PPCS protein on WB in HEK293 cells. Dilution used was 1:1000 and incubation was 2h at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mammalian cells