POU3F3-Specific Polyklonaler Antikörper

POU3F3-Specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18999-1-AP

Synonyme

Brain 1, Brn 1, BRN1, Oct 8, Octamer binding protein 8, OTF 8, OTF8, POU class 3 homeobox 3, POU3F3

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18999-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18999-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18999-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 18999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

18999-1-AP bindet in WB, IHC, ELISA POU3F3-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: POU class 3 homeobox 3
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: NM_006236
• Gene symbol: POU3F3
• Gene ID (NCBI): 5455
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTF8, also named as POU3F3 and BRN1, belongs to the POU transcription factor family and Class-3 subfamily. The antibody is specific to OTF8.

Publikationen

Species	Application	Title
mouse	WB	Dev Cell; AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.; Authors - Alexander G Marneros
human	WB	Int Immunopharmacol; Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling.; Authors - Faming Wang
human	IHC	Histochem Cell Biol; Connecting tubules develop from the tip of the ureteric bud in the human kidney.; Authors - Beatrix Sarkany