BRN2 Polyklonaler Antikörper

BRN2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 14596-1-AP

Synonyme

Brain 2, Brn 2, BRN2, Oct 7, OCT7, Octamer binding protein 7, OTF 7, OTF7, POU class 3 homeobox 2, POU3F2, POUF3

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 14596-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14596-1-AP (BRN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14596-1-AP (BRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human gliomas using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse brain using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Eierstockgewebe, humanes Gliomgewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, humanes Gliomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 4 publications below

Produktinformation

14596-1-AP bindet in WB, IHC, IF, ELISA BRN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRN2 fusion protein Ag6131
• Vollständiger Name: POU class 3 homeobox 2
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC051699
• Gene symbol: POU3F2
• Gene ID (NCBI): 5454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

Publikationen

Species	Application	Title
mouse	IF	Hum Mol Genet; Glutathione S-transferase Pi (Gstp) proteins regulate neuritogenesis in the developing cerebral cortex.; Authors - Xiaonan Liu
rat	WB	FEBS Lett; miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells.; Authors - Qian Liu
mouse	IF	Neurosci Lett; Overexpression of the 14-3-3gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex.; Authors - Brett Cornell
mouse	IF	Neuron; HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development.; Authors - Tianxiang Tang
mouse	IF	Adv Sci (Weinh); Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic Brain; Authors - Zhifu Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Mandi (Verified Customer) (09-26-2019)

Positive staining in human cortical neurons derived from iPSCs

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500