OCT2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OCT2-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18996-1-AP

Synonyme

OCT2, OCT2-Specific, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2

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Galerie der Validierungsdaten

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  WB analysis of mouse thymus using 18996-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18996-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18996-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18996-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 18996-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 18996-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18996-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, humanes Hirngewebe, humanes Milzgewebe, Mausnierengewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

18996-1-AP bindet in WB, IHC, ELISA OCT2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: POU class 2 homeobox 2
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 52-66 kDa
• GenBank-Zugangsnummer: NM_002698
• Gene symbol: POU2F2
• Gene ID (NCBI): 5452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).

Publikationen

Species	Application	Title
human	WB	Mol Ther; miR-3687 Overexpression Promotes Bladder Cancer Cell Growth by Inhibiting the Negative Effect of FOXP1 on Cyclin E2 Transcription.; Authors - Qipeng Xie
human,mouse	WB,IHC	Int J Oncol; Receptor for activated C kinase 1 promotes cervical cancer lymph node metastasis via the glycolysis‑dependent AKT/mTOR signaling.; Authors - Lixiu Xu
human	WB	Biochem Biophys Res Commun; CBX7 represses the POU2F2 to inhibit the PD-L1 expression and regulate the immune response in bladder cancer.; Authors - Weigang Ren ; KD Validated
	WB	Toxicol In Vitro; Comparisons of segment-specific toxicity of platinum-based agents and cadmium using S1, S2, and S3 cells derived from mouse kidney proximal tubules.; Authors - Hitomi Fujishiro
mouse	WB	Age (Dordr); Aging increases the susceptibility of cisplatin-induced nephrotoxicity.; Authors - Jiagen Wen