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POLR3B Polyklonaler Antikörper

POLR3B Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16574-1-AP

Synonyme

C128, POLR3B, RNA polymerase III subunit C2, RPC2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HepG2 cells using POLR3B Polyclonal antibody (16574-1-AP)

    WB analysis of HepG2 using 16574-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16574-1-AP (POLR3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using POLR3B Polyclonal antibody (16574-1-AP)

    WB analysis of HepG2 using 16574-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16574-1-AP (POLR3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below
IFSee 1 publications below

Produktinformation

16574-1-AP bindet in WB, IF, ELISA POLR3B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen POLR3B fusion protein Ag9856
Vollständiger Name polymerase (RNA) III (DNA directed) polypeptide B
Berechnetes Molekulargewicht 1133 aa, 128 kDa
Beobachtetes Molekulargewicht 128 kDa
GenBank-ZugangsnummerBC046238
Gene symbol POLR3B
Gene ID (NCBI) 55703
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for POLR3B antibody 16574-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB,IF

Cell Rep

Subcellular localization shapes the fate of RNA polymerase III

Authors - Kai Tian
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Krishnendu (Verified Customer) (02-27-2020)

Didn't work at all

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T