POLR2A Polyklonaler Antikörper

POLR2A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 20655-1-AP

Synonyme

hRPB220, hsRPB1, Pol II, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20655-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20655-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20655-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 20655-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

20655-1-AP bindet in WB, IHC, IF, ELISA POLR2A und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Berechnetes Molekulargewicht: 217 kDa
• Beobachtetes Molekulargewicht: 210-250 kDa
• GenBank-Zugangsnummer: NM_000937
• Gene symbol: POLR2A
• Gene ID (NCBI): 5430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice.; Authors - Yuanyuan Zhang
human	WB,IF	J Exp Clin Cancer Res; LncMAPK6 drives MAPK6 expression and liver TIC self-renewal.; Authors - Guanqun Huang
human	WB	Cell Death Discov; Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinoma; Authors - Yadong Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marta (Verified Customer) (02-28-2022)

Method: dot blot

• Applications: Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: glioma

POLR2A Antibody Other validation (1:1000 dilution) in glioma (Cat no:20655-1-AP)