POLR1E Polyklonaler Antikörper

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• KD/KO Validated

POLR1E Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16145-1-AP

Synonyme

PAF53, POLR1E, PRAF1, RNA polymerase I subunit A49, RP11 405L18.3

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Galerie der Validierungsdaten

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  WB analysis using 16145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16145-1-AP

  WB result of POLR1E antibody (16145-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR1E transfected MCF-7 cells.

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  WB analysis of human brain using 16145-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16145-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16145-1-AP

  IP Result of anti-POLR1E (IP:16145-1-AP, 3ug; Detection:16145-1-AP 1:500) with Jurkat cells lysate 1000ug.

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  IHC staining of human liver cancer using 16145-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16145-1-AP (POLR1E Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

16145-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA POLR1E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR1E fusion protein Ag9269
• Vollständiger Name: polymerase (RNA) I polypeptide E, 53kDa
• Berechnetes Molekulargewicht: 481aa,54 kDa; 419aa,47 kDa
• Beobachtetes Molekulargewicht: 47-53 kDa
• GenBank-Zugangsnummer: BC014331
• Gene symbol: POLR1E
• Gene ID (NCBI): 64425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	ChIP	Science; lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.; Authors - Man Wu
human	WB	J Biol Chem; Identification of an E3 ligase that targets the catalytic subunit of RNA polymerase I upon transcription stress; Authors - Stephanie Pitts
	WB	J Biol Chem; Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains.; Authors - Rachel McNamar
human	WB	Cancer Invest; Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells; Authors - Hu Lei
human	IF	PLoS One; Expression of RNA polymerase I catalytic core is influenced by RPA12; Authors - Brittany L Ford
human	IF	Mol Biol Cell; Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer; Authors - Aaron Rodrigues