DNA Polymerase Beta Polyklonaler Antikörper

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• KD/KO Validated

DNA Polymerase Beta Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18003-1-AP

Synonyme

DNA polymerase beta, POLB

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Galerie der Validierungsdaten

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  WB analysis using 18003-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18003-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18003-1-AP

  IP Result of anti-DNA polymerase beta (IP:18003-1-AP, 4ug; Detection:18003-1-AP 1:800) with K-562 cells lysate 3200ug.

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  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

18003-1-AP bindet in WB, IP, IHC, ELISA DNA Polymerase Beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNA Polymerase Beta fusion protein Ag12496
• Vollständiger Name: polymerase (DNA directed), beta
• Berechnetes Molekulargewicht: 335 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC100288
• Gene symbol: POLB
• Gene ID (NCBI): 5423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human		Proc Natl Acad Sci U S A; Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.; Authors - Shiladitya Sengupta
mouse	WB	Cancer Cell Int; Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.; Authors - Yuanyuan Wang ; KO Validated
human	WB	DNA Repair (Amst); Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.; Authors - Shiladitya Sengupta
human		NAR Cancer; XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells.; Authors - Bradley J Eckelmann