POFUT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

POFUT1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 14929-1-AP

Synonyme

FUT12, KIAA0180, O Fuc T, O FucT 1, O FUT, Peptide O fucosyltransferase 1, POFUT1, protein O fucosyltransferase 1

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Galerie der Validierungsdaten

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  WB analysis of HCT 116 using 14929-1-AP

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 14929-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14929-1-AP

  IP Result of anti-POFUT1 (IP:14929-1-AP, 4ug; Detection:14929-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 14929-1-AP (POFUT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 14929-1-AP (POFUT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 14929-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using POFUT1 antibody (14929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 14929-1-AP

  Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HUVEC-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HUVEC-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Produktinformation

14929-1-AP bindet in WB, IP, IHC, IF, ELISA POFUT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POFUT1 fusion protein Ag6710
• Vollständiger Name: protein O-fucosyltransferase 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000582
• Gene symbol: POFUT1
• Gene ID (NCBI): 23509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.

Publikationen

Species	Application	Title
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
human	WB,IHC,IF	EBioMedicine; PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.; Authors - Daojiang Li ; KD Validated
human	WB	EMBO Mol Med; A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder; Authors - Ren e G Feichtinger
human	WB,IHC,IF	Cell Death Dis; POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.; Authors - Yuheng Du ; KD Validated
human	IHC, IF	Cell Death Dis; LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.; Authors - S Liu
human	WB	Cell Prolif; Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.; Authors - Xinyuan Cui