PNPT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PNPT1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14487-1-AP

Synonyme

3 5 RNA exonuclease OLD35, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1

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Galerie der Validierungsdaten

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  WB analysis using 14487-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14487-1-AP (PNPT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14487-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14487-1-AP (PNPT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14487-1-AP

  IP Result of anti-PNPT1 (IP:14487-1-AP, 4ug; Detection:14487-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14487-1-AP (PNPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14487-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14487-1-AP (PNPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

14487-1-AP bindet in WB, IP, IHC, IF, ELISA PNPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PNPT1 fusion protein Ag5888
• Vollständiger Name: polyribonucleotide nucleotidyltransferase 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC053660
• Gene symbol: PNPT1
• Gene ID (NCBI): 87178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Publikationen

Species	Application	Title
human	WB	Immunity; Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis; Authors - Rui Miao ; KD Validated
mouse	WB,IHC	Am J Hum Genet; A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.; Authors - von Ameln Simon S ; KO Validated
mouse	IF	Proc Natl Acad Sci U S A; EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.; Authors - Tina Pangršič
mouse	WB	EMBO J; CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.; Authors - Karolina Szczepanowska
human	WB	PLoS Genet; RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer.; Authors - Mrinmoyee Majumder
mouse	IF	Front Cell Dev Biol; Differences in Calcium Clearance at Inner Hair Cell Active Zones May Underlie the Difference in Susceptibility to Noise-Induced Cochlea Synaptopathy of C57BL/6J and CBA/CaJ Mice.; Authors - Hongchao Liu