PML Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PML Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (2)

Kat-Nr. : 21041-1-AP

Synonyme

MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 21041-1-AP

  WB result of PML antibody (21041-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PML transfected HeLa cells.

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  WB analysis of HEK-293 using 21041-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21041-1-AP (PML Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21041-1-AP

  IP Result of anti-PML (IP:21041-1-AP, 4ug; Detection:21041-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human liver using 21041-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21041-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21041-1-AP (PML antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

21041-1-AP bindet in WB, IP, IHC, IF, ELISA PML und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PML fusion protein Ag15377
• Vollständiger Name: promyelocytic leukemia
• Berechnetes Molekulargewicht: 882 aa, 98 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC020994
• Gene symbol: PML
• Gene ID (NCBI): 5371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	IHC,IF	Theranostics; Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.; Authors - Wen Li
human	WB	Front Oncol; Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.; Authors - Shengye Liu
human	WB,IF	J Ginseng Res; 20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades.; Authors - Sirui Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

The antibody works really well in ICC in human cells (in 5% goat serum in PBST, ON incubation).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, U2OS cells

PML Antibody Immunofluorescence validation (1:1000 dilution) in Human, U2OS cells (Cat no:21041-1-AP)

FH

Eishi (Verified Customer) (11-17-2017)

Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein

PML Antibody Western Blot validation (1:500 dilution) in Protein (Cat no:21041-1-AP)